rHu F7 ;Factor VII heavy chain protein, N-His

rHu F7 ;Factor VII heavy chain protein, N-His

Concentration :  Lot depend

Species :  Human

AA Seq :  334-452/466

Predicted MW :  15.53 kDa

Tags :  N-His

Activity :  Not tested

Purity :  >90% as determined by SDS-PAGE.

Purification :  AC

Form :  Lyophilized or Liquid

Storage :  Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background:  Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins  in the coagulation cascade. It is an enzyme of the serine protease class. Factor VII contains   two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction

with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor

pathway inhibitor (TFPI), which is released almost immediately after initiation of

coagulation. Factor VII is vitamin K dependent and is produced in the liver. The use of

warfarin or similar anticoagulants impairs its function. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. The most important substrates for FVIIa-TF are Factor X and Factor IX.